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Published date: 2024/09/08
- Location: Dxb, Saint Matthews, Washington, D.C., United States
In order to determine if RMD controls F-actin organization within the pollen tube, laundry bags F-actin arrays in wild type and rmd-1 mature pollen grains were observed using Alexa Fluor 488-phalloidin staining. The maximum length of the F-actin cables were shorter in the rmd-mutant pollen tubes compared to those in the wild type tubes. Strongly bundled actin filaments were present around the apertures of the wild type pollen grains although there was no accumulation of actin filaments around the apertures in the rmd-1 pollen grains. But, there was a lower density of F-actin observed in the tip region of the rmd-mutant tubes compared to the wild type tubes. RMD, which are type II formins, consist of a phosphatase, (PTEN)-like domain (responsible for protein localization), and FH1 and FH2 domains (promotes actin polymerization). A type of parsley not carried here previously -- perfect!
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