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Published date: 2024/08/17
- Location: Ky8 3ad, Quimby, Minnesota, United States
Whole cell lysate (20 μl) was combined with 80 μl of 0.05 N H2SO4 (hydrolysis reagent) and incubated at 80°C for 60 min. If you have almost any queries concerning in which along with the best way to utilize Supplier of sialic acid powder as Raw Material for Supplements, you'll be able to call us from our own web-site. Samples were briefly centrifuged at 14,000 revolution/min (16,000 g), after which 20 μl of 1 M NaOH (neutralization reagent) was added and the mixture centrifuged again at 14,000 revolution/min. Afterwards, wells were washed 3 times, 100 µl of substrate solution was added for 30 min at RT and the optical density (OD) was measured at 405 nm by an ELISA reader (TECAN Infinite 200 PRO).
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