Preparation Of Buffer Solutions (Phosphate, Acetate And Different Buffers)
45.00 £
Published date: 2025/03/02
- Location: 8724, Cane Savannah, North Dakota, United States
Acetic Ammonia Buffer pH 3.7, Ethanolic: To 15 ml of 5 M acetic acid add 60 ml of ethanol (95 p.c) and 24 ml of water. Regulate the pH to three.7 with 10 M ammonia and dilute with water to 100 ml. Acetone Answer, Buffered: Dissolve eight. 15 g of sodium acetate and MAP CAS 7722-76-1 ， forty two g of sodium chloride in water, add 68 ml of 0.1 M hydrochloric acid and one hundred fifty ml of acetone and dilute with water to 500 ml. Buffered Saline pH 7.2: Dissolve 10.75 g of disodium hydrogen phosphate, 7.6 g of sodium chloride and 10 g of bovine albumin in adequate water to produce 1000 ml. Earlier than use modify to pH 7.2 with 2 M sodium hydroxide or a 10 % w/v solution of phosphoric acid as required. Ammonia-Ammonium Chloride Buffer: Dissolve 67.5 g of ammonium chloride in about 200 ml of water, add 570 ml of strong ammonia solution and dilute with water to a thousand ml. Ammonia Buffer pH 9.5: Dissolve 33.
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