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Preparation Of Buffer Solutions (Phosphate, Acetate And Other Buffers)

232.00 $
Published date: 2025/03/02
  • Location: 85000, Gypsy, Alabama, United States
Acetic Ammonia Buffer pH 3.7, Ethanolic: To 15 ml of 5 M acetic acid add 60 ml of ethanol (ninety five p.c) and 24 ml of water. Adjust the pH to 3.7 with 10 M ammonia and dilute with water to one hundred ml. Acetone Answer, Buffered: Dissolve eight. 15 g of sodium acetate and forty two g of sodium chloride in water, add 68 ml of zero.1 M hydrochloric acid and one hundred fifty ml of acetone and dilute with water to 500 ml. Buffered Saline pH 7.2: Dissolve 10.Seventy five g of Disodium Hydrogen Phosphate Dodecahydrate hydrogen phosphate, 7.6 g of sodium chloride and 10 g of bovine albumin in sufficient water to supply 1000 ml. Before use alter to pH 7.2 with 2 M sodium hydroxide or a ten % w/v solution of phosphoric acid as required. Ammonia-Ammonium Chloride Buffer: Dissolve 67.5 g of ammonium chloride in about 200 ml of water, add 570 ml of strong ammonia resolution and dilute with water to 1000 ml. Ammonia Buffer pH 9.5: Dissolve 33.

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