US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents
169.00 $
Published date: 2024/09/21
- Location: 6998 An, Nebraska, United States
In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. Here's more in regards to manufacturer of sialic acid powder as Raw Material for food look at the web-page. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7.8, dialyzed against assay buffer (20 mm Tris, 150 mm NaCl, 2 mm tris(2-carboxyethyl)phosphine, pH 7.5), and finally concentrated to 0.5-1 mm for storage at 4 °C. All 1D NMR experiments were performed using a Bruker Advance I 500-MHz spectrometer with a 5-mm PATXI 1H/D-13C/15N Z-GRD probe at 293 K.
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